Human castrate-resistant prostate cancer cell line
cell line
VCaP
chip antibody
GATA2 antibody (Sigma, HPA005633)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells with fixed with 1% formaldehyde and lysed to extract chromatin. Chromatin was sheared using sonication. DNA-protien complexes were immunoprecipitatied using an anti-GATA2 antibody (Sigma, HPA005633) Indexed libraries were prepared from 5 ng of ChIP DNA with TruSeq ChIP Sample Prep Kit (Illumina) according to the manufacturer's instructions. Libraries were quantified using the Tape Station 2200 (Agilent Technologies) and Qubit Fluorometer 1.0 (Invitrogen Co., Carlsbad, CA). Libraries were pooled such that each index-tagged sample was present in equimolar amounts, with final concentration of the pooled samples of 2nM. The pooled samples were subject to cluster generation and sequencing using an Illumina NextSeq 500 System (Illumina) in a 1x75 single-end format