GSM3564520: PLZF d0: PLZF naïve ChIP; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
ZBTB16
Cell type
Cell type Class
Others
Cell type
Mesenchymal stem cells
NA
NA
Attributes by original data submitter
Sample
source_name
Bone marrow derived hMSC
cell type
human Mesenchymal Stem Cells
passages
p4-p10
chip antibody
anti PLZF-SantaCruz (sc-22839)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were processed for ChIP as previously described with slight modifications (Dietrich, Lerdrup et al., 2012). Briefly, cells were fixed for 10 min in culture media containing 1% formaldehyde and were processed for ChIP as previously described with slight modifications (Dietrich et al., 2012). ChIP DNA from three parallel ChIPs were pooled or individual ChIPs from three biological replicates were used and 10 ng each was used for making ChIP-seq libraries. The libraries were prepared using “ChIP seq DNA sample preparation kit” from Illumina following manufacturer's instructions. H3K27ac samples were multiplexed (using NEB kit) and run on HiSeq2000 (Illumina).