Cells were treated with DMEM with low pH (~pH 6.7; 0.37 g/L sodium bicarbonate).
cell line
HeLa
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked with 1% formaldehyde for 10 min at room temperature and then were treated with 0.125M glycine for 5 min to stop cross-linking reaction. Cross-linked cells were washed with 1X cold PBS and scraped for harvest. MondoA-DNA complexes were isolated using MondoA antibody (Proteintech). ChIP library was generated as described in Reddy et al. 2009.