Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MLXIP

Cell type

Cell type Class
Uterus
Cell type
HeLa
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
HeLa cells
chip antibody
MLXIP Rabbit Polyclonal Antibody (Proteintech; 13614-1-AP)
treatment
Cells were treated with DMEM with low pH (~pH 6.7; 0.37 g/L sodium bicarbonate).
cell line
HeLa

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked with 1% formaldehyde for 10 min at room temperature and then were treated with 0.125M glycine for 5 min to stop cross-linking reaction. Cross-linked cells were washed with 1X cold PBS and scraped for harvest. MondoA-DNA complexes were isolated using MondoA antibody (Proteintech). ChIP library was generated as described in Reddy et al. 2009.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
18490291
Reads aligned (%)
63.2
Duplicates removed (%)
22.1
Number of peaks
2130 (qval < 1E-05)

hg38

Number of total reads
18490291
Reads aligned (%)
64.5
Duplicates removed (%)
21.1
Number of peaks
2158 (qval < 1E-05)

Base call quality data from DBCLS SRA