Cells were treated with DMEM with normal pH (~pH 7.2; 3.7 g/L sodium bicarbonate)
Sequenced DNA Library
Cells were crosslinked with 1% formaldehyde for 10 min at room temperature and then were treated with 0.125M glycine for 5 min to stop cross-linking reaction. Cross-linked cells were washed with 1X cold PBS and scraped for harvest. MondoA-DNA complexes were isolated using MondoA antibody (Proteintech). ChIP library was generated as described in Reddy et al. 2009.