Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pluripotent stem cell
Cell type
iPSC derived neural cells
NA
NA

Attributes by original data submitter

Sample

source_name
Neural stem cells
cell type
CD34+ HSPC-derived iPSC-differentiated NSC
passage
12-15
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Total genomic DNA was fragmented to 200-500bp with a Covaris water-bath sonicator. DNA was purified and eluted using a PCR purification kit (Qiagen) in 30 μl HPLC-grade water. ChIP-Seq libraries were prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
39838679
Reads aligned (%)
95.3
Duplicates removed (%)
17.3
Number of peaks
5411 (qval < 1E-05)

hg19

Number of total reads
39838679
Reads aligned (%)
95.1
Duplicates removed (%)
17.6
Number of peaks
5075 (qval < 1E-05)

Base call quality data from DBCLS SRA