GSM1372579: ES cell d7 Input DNA Rep1; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Pluripotent stem cell
Cell type
mESCs, differentiated
NA
NA
Attributes by original data submitter
Sample
source_name
Day 7 differentiated mouse embryonic stem cells
Sex
female
strain
F2: 129S1/CastEiJ x 129S1
cell type
Day 7 differentiated mouse embryonic stem cells
antibody
None
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were sonicated in a covaris S2, precleared with protein G agarose and protein-DNA complexes were precipitated using specific antibodies. Paired end ChIP-sequencing library preparation kit (E6240S) and Illumina multiplexing oligos (E7335S) were purchased from New England Biolabs. Libraries were quantified on a Bioanalyzer using the Agilent high sensitivity DNA kit (Agilent Technologies Cat. No. 5067-4627) and by qPCR using the KAPA library quantification kit (KAPA Biosystems Inc Cat. No. KK4824). Pooled samples were sequenced using Illumina HiSeq 2000 resulting in approximately 25-50 million of 50 bp paired-end reads per sample.