anti-DP (rabbit, #212, Dimova et al., Genes Dev. 2003;17: 2308-2320)
developmental stage
Pharate stage (96h APF at 5 degrees C)
genotype
P{UAS-mCherry-RNAi}attP2 ; P{GAL4-Mef2.R}3
tissue
flight muscles
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin from flight muscles was extracted using homogenizer and fixed in 1.8% formaldehyde. Then lysates were sheared using a Sonifier, and chromatin was immunoprecipitated with antibodies as in Zappia, M. P. and Frolov, M. V. Nat. Commun. 7:10509 (2016) ChIPSeq libraries were generated using an Illumina ChIP-SEQ kit