Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Dp1

Cell type

Cell type Class
Adult
Cell type
Flight muscles
NA
NA

Attributes by original data submitter

Sample

source_name
flight muscles
chip antibody
anti-DP (rabbit, #212, Dimova et al., Genes Dev. 2003;17: 2308-2320)
developmental stage
Pharate stage (96h APF at 5 degrees C)
genotype
P{UAS-mCherry-RNAi}attP2 ; P{GAL4-Mef2.R}3
tissue
flight muscles

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin from flight muscles was extracted using homogenizer and fixed in 1.8% formaldehyde. Then lysates were sheared using a Sonifier, and chromatin was immunoprecipitated with antibodies as in Zappia, M. P. and Frolov, M. V. Nat. Commun. 7:10509 (2016) ChIPSeq libraries were generated using an Illumina ChIP-SEQ kit

Sequencing Platform

instrument_model
Illumina HiSeq 2500

dm3

Number of total reads
42375457
Reads aligned (%)
13.8
Duplicates removed (%)
70.0
Number of peaks
4168 (qval < 1E-05)

dm6

Number of total reads
42375457
Reads aligned (%)
12.5
Duplicates removed (%)
72.7
Number of peaks
3977 (qval < 1E-05)

Base call quality data from DBCLS SRA