Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MYC

Cell type

Cell type Class
Breast
Cell type
UACC-812
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal

Attributes by original data submitter

Sample

source_name
tumor cell
cell line
UACC-812 C-MYC overexpression cell line
tissue
breast
chip antibody
CMYC

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
A total of 1 × 106 cells were fixed in 1% formaldehyde at room temperature for 10 min, and the nuclei were isolated with nuclear lysis buffer supplemented with a protease inhibitor. Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (Part# 0801-0303).

Sequencing Platform

instrument_model
HiSeq X Ten

hg19

Number of total reads
7472509
Reads aligned (%)
52.7
Duplicates removed (%)
10.0
Number of peaks
103 (qval < 1E-05)

hg38

Number of total reads
7472509
Reads aligned (%)
53.8
Duplicates removed (%)
8.8
Number of peaks
80 (qval < 1E-05)

Base call quality data from DBCLS SRA