Embryonic heart and hindbrain was isolated from CD1 mouse embryos at e11.5 .Tissue samples were cross linked and cells were dissociated and subject to chromatin isolation, sonication and immunoprecipitation. ChIP DNA was sheared by sonication, end-repaired, ligated to illumina sequencing adapters. ChIP and input DNA between 300 and 500bp was sequenced on the Illumina HiSeq 2000 to generate 50 or 36bp reads.