Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me3

Cell type

Cell type Class
Embryo
Cell type
Embryonic limb
NA
NA

Attributes by original data submitter

Sample

source_name
Mouse embryonic day 11.5 (e11.5) limb
developmental stage
e11.5
tissue
limb
chip antibody
H3K27me3
strain
CD1
Sex
pooled male and female

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Embryonic heart and hindbrain was isolated from CD1 mouse embryos at e11.5 .Tissue samples were cross linked and cells were dissociated and subject to chromatin isolation, sonication and immunoprecipitation. ChIP DNA was sheared by sonication, end-repaired, ligated to illumina sequencing adapters. ChIP and input DNA between 300 and 500bp was sequenced on the Illumina HiSeq 2000 to generate 50 or 36bp reads.

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

mm10

Number of total reads
32957874
Reads aligned (%)
93.8
Duplicates removed (%)
13.8
Number of peaks
11677 (qval < 1E-05)

mm9

Number of total reads
32957874
Reads aligned (%)
93.7
Duplicates removed (%)
13.9
Number of peaks
11708 (qval < 1E-05)

Base call quality data from DBCLS SRA