Antigen

Antigen Class

TFs and others

Antigen

BRD4

Cell type

Cell type Class

Breast

Cell type

HCC1806

Primary Tissue

Breast

Tissue Diagnosis

Carcinoma Squamous Cell

Sample

source_name

breast cancer

cell line

HCC1806

chip antibody

BRD4, A301-985A50

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

HCC1806 and HCC1937 cells were cross-linked with 1 % formaldehyde (for H3K27Ac), or cross-linked with disuccinimidyl glutarate, followed by double cross-linked with 1 % formaldehyde (for BRD4). The libraries were constructed following NEB's Chip-Seq Sample prep kit. Briefly, Chip DNA was end-blunted and added with an 'A' base so the adaptors from Illumina with a 'T' can ligate on the ends. Then 200–400 bp fragments are gel-isolated and purified. The library was amplified by 18 cycles of PCR.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

29820092

Reads aligned (%)

97.2

Duplicates removed (%)

4.7

Number of peaks

1077 (qval < 1E-05)

hg19

Number of total reads

29820092

Reads aligned (%)

96.5

Duplicates removed (%)

6.2

Number of peaks

1030 (qval < 1E-05)