GSM3553094: ChIP-Seq HCC1806 BRD4; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
TFs and others
Cell type Class
Carcinoma Squamous Cell
Attributes by original data submitter
Sequenced DNA Library
HCC1806 and HCC1937 cells were cross-linked with 1 % formaldehyde (for H3K27Ac), or cross-linked with disuccinimidyl glutarate, followed by double cross-linked with 1 % formaldehyde (for BRD4). The libraries were constructed following NEB's Chip-Seq Sample prep kit. Briefly, Chip DNA was end-blunted and added with an 'A' base so the adaptors from Illumina with a 'T' can ligate on the ends. Then 200–400 bp fragments are gel-isolated and purified. The library was amplified by 18 cycles of PCR.