Antigen

Antigen Class

TFs and others

Antigen

SMARCA4

Cell type

Cell type Class

Others

Cell type

Mel270

NA

NA

Sample

source_name

MEL270

cell line

MEL270

lentiviral vector

pINDUCER-FLAG-SF3B1_WT

chip antibody

BRG1 / SMARCA4 (Abcam EPNCIR111A)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were fixed in 1% formaldehyde (Sigma Aldrich, F8775) for 10min at 37C and quenched with 125mM glycine for 5min at 37C. After washing, nuclei were sonicated using Covaris Sonicator, and the supernatant was used for immunoprecipitation with the indicated antibody. ChIP-seq libraries were prepared with the Rubicon Thruplex DNAseq prep kit using standard protocols

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

19408244

Reads aligned (%)

94.5

Duplicates removed (%)

18.1

Number of peaks

45262 (qval < 1E-05)

hg19

Number of total reads

19408244

Reads aligned (%)

94.2

Duplicates removed (%)

18.5

Number of peaks

46112 (qval < 1E-05)