Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BRD9

Cell type

Cell type Class
Others
Cell type
Mel270
NA
NA

Attributes by original data submitter

Sample

source_name
MEL270
cell line
MEL270
lentiviral vector
pINDUCER-FLAG-SF3B1_WT
chip antibody
BRD9 (Abcam ab137245)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed in 1% formaldehyde (Sigma Aldrich, F8775) for 10min at 37C and quenched with 125mM glycine for 5min at 37C. After washing, nuclei were sonicated using Covaris Sonicator, and the supernatant was used for immunoprecipitation with the indicated antibody. ChIP-seq libraries were prepared with the Rubicon Thruplex DNAseq prep kit using standard protocols

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
15700266
Reads aligned (%)
64.3
Duplicates removed (%)
27.1
Number of peaks
5921 (qval < 1E-05)

hg38

Number of total reads
15700266
Reads aligned (%)
67.4
Duplicates removed (%)
24.6
Number of peaks
5864 (qval < 1E-05)

Base call quality data from DBCLS SRA