GSM3544068: MEL270.SF3B1 K700E.input; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Others
Cell type
Mel270
NA
NA
Attributes by original data submitter
Sample
source_name
MEL270
cell line
MEL270
lentiviral vector
pINDUCER-FLAG-SF3B1_K700E
chip antibody
None
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed in 1% formaldehyde (Sigma Aldrich, F8775) for 10min at 37C and quenched with 125mM glycine for 5min at 37C. After washing, nuclei were sonicated using Covaris Sonicator, and the supernatant was used for immunoprecipitation with the indicated antibody. ChIP-seq libraries were prepared with the Rubicon Thruplex DNAseq prep kit using standard protocols