Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SMARCA4

Cell type

Cell type Class
Others
Cell type
Mel270
NA
NA

Attributes by original data submitter

Sample

source_name
MEL270
cell line
MEL270
lentiviral vector
pINDUCER eGFP empty
chip antibody
BRG1 / SMARCA4 (Abcam EPNCIR111A)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed in 1% formaldehyde (Sigma Aldrich, F8775) for 10min at 37C and quenched with 125mM glycine for 5min at 37C. After washing, nuclei were sonicated using Covaris Sonicator, and the supernatant was used for immunoprecipitation with the indicated antibody. ChIP-seq libraries were prepared with the Rubicon Thruplex DNAseq prep kit using standard protocols

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
21373496
Reads aligned (%)
82.3
Duplicates removed (%)
13.0
Number of peaks
54759 (qval < 1E-05)

hg38

Number of total reads
21373496
Reads aligned (%)
82.8
Duplicates removed (%)
12.7
Number of peaks
53822 (qval < 1E-05)

Base call quality data from DBCLS SRA