Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Breast
Cell type
BT-549
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal

Attributes by original data submitter

Sample

source_name
BT-549 cells
cell line
BT-549 (HTB-122)
treatment
control
chip antibody
n/a

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was immunoprecipitated using RNA Pol II antibody, washed, reverse-crosslinked, and DNA isolated using the phenol-chloroform method. Input DNA isolated using the phenol-chloroform method. 10ng precipitated DNA was used to prepare library using Illumina TruSeq ChIP library preparation kit.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
28619001
Reads aligned (%)
98.0
Duplicates removed (%)
1.7
Number of peaks
580 (qval < 1E-05)

hg19

Number of total reads
28619001
Reads aligned (%)
97.1
Duplicates removed (%)
2.0
Number of peaks
329 (qval < 1E-05)

Base call quality data from DBCLS SRA