For ChIP-seq, the HEK293-GR cells were grown on 10-cm dishes in charcoal-stripped medium for 2 days, treated with vehicle or dex (100 nM, 1 h), and fixed with 1% (v/v) formaldehyde 10 min at room temperature. Chromatin was fragmented to ∼200–400 bp using sonication (Bioruptor UCD-300, Diagenode). Anti-IRF2BP2 antibody (A303190A, Bethyl laboratories) was coupled to protein-A- beads (Millipore), fragmented chromatin was incubated with antibody-coupled beads overnight, washed, eluted and de-crosslinked in the presence of proteinase K (Fermentas). Chromatin fragments were purified using MinElute columns (Qiagen). Fragmented de-crosslinked chromatin was used as an input control. Library preparation was done using NEBNext Ultra II kit (New England Biolabs) and sequenced using Illumina Hiseq2000 at EMBL GeneCore (Heidelberg, Germany).