GSM3537880: H9-NPC-UBE3A-351-2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
UBE3A
Cell type
Cell type Class
Pluripotent stem cell
Cell type
hESC derived neural cells
NA
NA
Attributes by original data submitter
Sample
source_name
Neural progenitor cells
cell type
H9 hESC-derived neural progenitors
weeks of differentiation
5 weeks
genotype
wildtype
antibody
anti-UBE3A
antibody catalog number and manufacturer
A300-351A (Bethyl)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
hESCs, NPCs, and neurons were processed for ChIP-seq as previously described (Cotney & Noonan (2015), Cold Spring Harb Protoc 2015(4): 419 Libraries were prepared from ChIPed DNA using the Takara ThruPLEX DNA-seq library preparation kit according to manufacturer's instructions. Libraries were quantified with the NEBNext Library Quant Kit for Illumina