GSM1370513: HDAC1 in Arid3a OE cells Mock; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
source_name
embryonic stem cells
cell type
mouse embryonic stem cell
cell line
J1 ES cells containing BirA
chip antibody
None
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The cells were cross-linked with 1% formaldehyde, followed by sonication to solubilize the chromatin. The chromatin extracts containing DNA fragments with an average size of 500bp to 1Kb were immunoprecipitated using different antibodies/beads. ChIP-sequencing libraries were generated using NEB ChIP-seq library prep kit (E6200L) according to the manufacturer’s instructions.