GSM3532117: SW1990 BACH1 ChIPseq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
BACH1
Cell type
Cell type Class
Pancreas
Cell type
SW 1990
Primary Tissue
Pancreas
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
SW1990
cell line
Pancreatic ductal adenocarcinoma cell line SW1990
chip antibody
Bach1 (House made (A1-6))
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed 1% formaldehyde, followed by quenching with 125mM of glycine. The cells were washed with ice-cold PBS and were lysed. The crosslinked chromatin was shared with a Bioruptor Sonicator (CosmoBio) and target protein-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared from ~10 ng of ChIP DNA with the use of Ovation Ultralow DR Multiplex System (Nugen). The DNA libraries were clonally amplified on a flow cell and sequenced on HiSeq2500 (HiSeq Control Software v2.2.38, Illumina) with 51-mer paired-end sequence. Image analysis and base calling were performed using Real-Time Analysis Software (v1.18.61, Illumina).