Antigen

Antigen Class

TFs and others

Antigen

5-mC

Cell type

Cell type Class

Breast

Cell type

MCF 10A

Primary Tissue

Breast

Tissue Diagnosis

Fibrocystic Disease

Sample

source_name

Human breast cells

cell type

MCF10A

chip antibody

5metC

antibody vendor

Diagenode

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Performed with Illumina protocol. 10Ng of inmunoprecipitated DNA was en-repaired, and Illumina double stranded adapters for single read sequencing were ligated to the DNA. After purification of the sample with a MinElute spin column, we size the selected libraries on a 2% agarose gel and fragments corresponding to an insert size range of around 130bp were recovered. The libraries were finally amplified with an 18-cycle PCR reaction, and library quality was confirmed on the Agilent 2100 Bioanalyzer. The samples were loaded at concentrations of 8-11 pm onto Illumina single read flowcells and sequenced on the Illumina Genome Analyzer Iix using a 40 cycles recipe.

Sequencing Platform

instrument_model

Illumina Genome Analyzer IIx

hg38

Number of total reads

29331671

Reads aligned (%)

91.5

Duplicates removed (%)

5.7

Number of peaks

10676 (qval < 1E-05)

hg19

Number of total reads

29331671

Reads aligned (%)

90.7

Duplicates removed (%)

7.4

Number of peaks

10515 (qval < 1E-05)