Antigen

Antigen Class

TFs and others

Antigen

CBX5

Cell type

Cell type Class

Breast

Cell type

MCF-7

Primary Tissue

Breast

Site of Extraction

Pleura

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

Human breast cells

cell type

MCF7

chip antibody

HP1_alpha

antibody vendor

Abcam

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Performed with Illumina protocol. 10Ng of inmunoprecipitated DNA was en-repaired, and Illumina double stranded adapters for single read sequencing were ligated to the DNA. After purification of the sample with a MinElute spin column, we size the selected libraries on a 2% agarose gel and fragments corresponding to an insert size range of around 130bp were recovered. The libraries were finally amplified with an 18-cycle PCR reaction, and library quality was confirmed on the Agilent 2100 Bioanalyzer. The samples were loaded at concentrations of 8-11 pm onto Illumina single read flowcells and sequenced on the Illumina Genome Analyzer Iix using a 40 cycles recipe.

Sequencing Platform

instrument_model

Illumina Genome Analyzer IIx

hg38

Number of total reads

10697452

Reads aligned (%)

85.5

Duplicates removed (%)

30.7

Number of peaks

1230 (qval < 1E-05)

hg19

Number of total reads

10697452

Reads aligned (%)

84.1

Duplicates removed (%)

31.9

Number of peaks

1174 (qval < 1E-05)