Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me1

Cell type

Cell type Class
Breast
Cell type
4175-LM2
NA
NA

Attributes by original data submitter

Sample

source_name
LM2-4175 cell line
tissue
breast cancer cell line
cell line
LM2-4175
chip antibody
H3K4me1
antibody vendorname
Abcam
antibody vendorid
#8895
treatment
None

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For chromatin immunoprecipitation, MDA-MB-231 and LM2-4175 cells were harvested and performed by ChIP-IT High Sensitivity kit (Active Motif, #53040) according to manufacturer's instructions Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (Part# 0801-0303)

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
36199740
Reads aligned (%)
98.8
Duplicates removed (%)
5.7
Number of peaks
399 (qval < 1E-05)

hg19

Number of total reads
36199740
Reads aligned (%)
98.7
Duplicates removed (%)
5.8
Number of peaks
446 (qval < 1E-05)

Base call quality data from DBCLS SRA