Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SIPA1

Cell type

Cell type Class
Breast
Cell type
MDA-MB-231
Primary Tissue
Breast
Site of Extraction
Effusion, Pleural
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
breast cancer cell
cell line
MDA-MB-231 cell line
chip antibody
SIPA1 (Santa Cruz SC-137043)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and SIPA1-DNA complexes were isolated with antibody. Libraries were prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
25691031
Reads aligned (%)
18.0
Duplicates removed (%)
16.4
Number of peaks
953 (qval < 1E-05)

hg38

Number of total reads
25691031
Reads aligned (%)
18.2
Duplicates removed (%)
16.2
Number of peaks
912 (qval < 1E-05)

Base call quality data from DBCLS SRA