Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Neural
Cell type
Hippocampus
MeSH Description
A curved elevation of GRAY MATTER extending the entire length of the floor of the TEMPORAL HORN of the LATERAL VENTRICLE (see also TEMPORAL LOBE). The hippocampus proper, subiculum, and DENTATE GYRUS constitute the hippocampal formation. Sometimes authors include the ENTORHINAL CORTEX in the hippocampal formation.

Attributes by original data submitter

Sample

source_name
mouse hippocampus
genotype
control
chip antibody
input
strain
C57BL/6J genetic background
tissue
mouse hippocampus
Sex
male
age
adult 2.5 month old

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody against H3K27me3 (abcam, #ab6002). Libraries were prepared according to standard Illumina's protocols and sequenced on HiSeq 2500 apparatus following the manufacturer's protocols.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
20701750
Reads aligned (%)
99.0
Duplicates removed (%)
12.2
Number of peaks
353 (qval < 1E-05)

mm9

Number of total reads
20701750
Reads aligned (%)
98.8
Duplicates removed (%)
12.3
Number of peaks
369 (qval < 1E-05)

Base call quality data from DBCLS SRA