GSM3521657: Normal KDM6A ChIPSeq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
KDM6A
Cell type
Cell type Class
Blood
Cell type
Nuclear cells
NA
NA
Attributes by original data submitter
Sample
source_name
Normal peripheral blood nuclear cells
tissue
Peripheral blood
chip antibody
KDM6A (Bethyl Laboratories, Cat. A302-374A)
cell type
peripheral blood nuclear cells
disease state
normal
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were precipitated with antibody. ChIP-seq library preparation was performed using TruSeqChIP Sample Prep Kit (Illumina) according to the manufacturer's instructions.10ng of input ChIP-enriched DNA was used for ChIP-seq library preparation. Final libraries were checked using High Sensitivity chips in 2100 Bioanalyzer (Agilent). Average fragment size of final libraries was found to be 280 ± 8bp. Paired-end sequencing (2 x 100 bp) of these libraries were performed in HiSeq-2500 (Illumina) according to the manufacturer's protocols.