Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
CEBPA

Cell type

Cell type Class
Blood
Cell type
THP-1
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
Culture
cell line
THP-1
treatment
1,25(OH)2D3
time point
24h
chip antibody
anti-CEBPA (Diagenode, catalog number: C15410225)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were grown in culture as mentioned above, after which they were crosslinked with formaldehyde, lysed and the DNA was extracted. Then, ChIPmentation was performed. The library was created using NEBNext Ultra II DNA Library Prep Kit for Illumina and the samples were barcoded using Nextera sequencing primers. The pooled libraries were sequenced on an Illumina HiSeq2000.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
22444625
Reads aligned (%)
85.0
Duplicates removed (%)
13.1
Number of peaks
8211 (qval < 1E-05)

hg38

Number of total reads
22444625
Reads aligned (%)
87.0
Duplicates removed (%)
11.9
Number of peaks
8477 (qval < 1E-05)

Base call quality data from DBCLS SRA