GSM3519060: THP-1 2h after 1,25D treatment, 3rd replicate CEBPA; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
CEBPA
Cell type
Cell type Class
Blood
Cell type
THP-1
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous
Attributes by original data submitter
Sample
source_name
Culture
cell line
THP-1
treatment
1,25(OH)2D3
time point
2h
chip antibody
anti-CEBPA (Diagenode, catalog number: C15410225)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were grown in culture as mentioned above, after which they were crosslinked with formaldehyde, lysed and the DNA was extracted. Then, ChIPmentation was performed. The library was created using NEBNext Ultra II DNA Library Prep Kit for Illumina and the samples were barcoded using Nextera sequencing primers. The pooled libraries were sequenced on an Illumina HiSeq2000.