Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me3

Cell type

Cell type Class
Blood
Cell type
CUTLL1
NA
NA

Attributes by original data submitter

Sample

source_name
ChIP-SEQ_H3K27me3_CUTLL1_shRENILLA
cell line
CUTLL1
cell type
human T cell leukemia cells
infected with
retroviruses expressing short haiprins against Renilla
chip antibody
H3K27me3
chip antibody vendor
abcam

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Micrococcal nuclease (MNASE) treated chromatin was used for the ChIP studies in an adaptation of the Upstate ChIP protocol Libraries were generated as described before, including end-repair, A-tailing, adapter (Illumina Truseq system) ligation and PCR amplification of the libraries.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
30725176
Reads aligned (%)
99.2
Duplicates removed (%)
29.8
Number of peaks
477 (qval < 1E-05)

hg19

Number of total reads
30725176
Reads aligned (%)
98.4
Duplicates removed (%)
30.5
Number of peaks
314 (qval < 1E-05)

Base call quality data from DBCLS SRA