Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Neural
Cell type
Medulloblastoma
MeSH Description
A malignant neoplasm that may be classified either as a glioma or as a primitive neuroectodermal tumor of childhood (see NEUROECTODERMAL TUMOR, PRIMITIVE). The tumor occurs most frequently in the first decade of life with the most typical location being the cerebellar vermis. Histologic features include a high degree of cellularity, frequent mitotic figures, and a tendency for the cells to organize into sheets or form rosettes. Medulloblastoma have a high propensity to spread throughout the craniospinal intradural axis. (From DeVita et al., Cancer: Principles and Practice of Oncology, 5th ed, pp2060-1)

Attributes by original data submitter

Sample

source_name
Input ChIP-seq control derived from pooled murine MGB (Myc/Gfi1b) medulloblastoma tumor
mouse strain
C57BL/6
tissue type
murine medulloblastoma tumor

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
To isolate NSCs, Prominin1+, Lineage-negative (lin-) cells were FACS-sorted from cerebella of 5-7-day-old WT mice. Tumor cells were isolated by enzymatic dissociation of cerebella from adult tumor-bearing mice. All cells were snap-frozen in liquid nitrogen. Frozen tumor tissue was processed for ChIP at ActiveMotif (Carlsbad, CA) according to proprietary protocols. According to Illumina protocols.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
48263593
Reads aligned (%)
0.0
Duplicates removed (%)
1.2
Number of peaks
471 (qval < 1E-05)

mm9

Number of total reads
48263593
Reads aligned (%)
0.0
Duplicates removed (%)
1.2
Number of peaks
416 (qval < 1E-05)

Base call quality data from DBCLS SRA