Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SPI1

Cell type

Cell type Class
Blood
Cell type
Dendritic Cells
MeSH Description
ANTIGEN-PRESENTING CELLS of dendritic cell morphology found in the LYMPH NODES and other lymphoid tissues.

Attributes by original data submitter

Sample

source_name
human inflammatory DC LPS stimulated
culture type
monocyte derived DC
cell type
dendritic cells (DC)
treatment and collection date
LPS added at day 7, collection day 10
culture conditions
GM-CSF + IL4
antibody
PU.1 (T-21, Santa Cruz)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
After crosslinking the chromatin of 2x10E8 cell lysates, sonicated nuclei and PU.1-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions.

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

hg19

Number of total reads
18576938
Reads aligned (%)
39.3
Duplicates removed (%)
57.0
Number of peaks
9699 (qval < 1E-05)

hg38

Number of total reads
18576938
Reads aligned (%)
41.1
Duplicates removed (%)
55.4
Number of peaks
9636 (qval < 1E-05)

Base call quality data from DBCLS SRA