Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SPI1

Cell type

Cell type Class
Blood
Cell type
Dendritic Cells
MeSH Description
ANTIGEN-PRESENTING CELLS of dendritic cell morphology found in the LYMPH NODES and other lymphoid tissues.

Attributes by original data submitter

Sample

source_name
human inflammatory DC immature
culture type
monocyte derived DC
cell type
dendritic cells (DC)
treatment and collection date
no LPS added, collection day 10
culture conditions
GM-CSF + IL4
antibody
PU.1 (T-21, Santa Cruz)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
After crosslinking the chromatin of 2x10E8 cell lysates, sonicated nuclei and PU.1-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions.

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

hg19

Number of total reads
15696736
Reads aligned (%)
76.0
Duplicates removed (%)
20.0
Number of peaks
823 (qval < 1E-05)

hg38

Number of total reads
15696736
Reads aligned (%)
79.1
Duplicates removed (%)
17.9
Number of peaks
677 (qval < 1E-05)

Base call quality data from DBCLS SRA