GSM3499389: MCF10A deltaHD CHIP seq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
SATB1
Cell type
Cell type Class
Breast
Cell type
MCF 10A
Primary Tissue
Breast
Tissue Diagnosis
Fibrocystic Disease
Attributes by original data submitter
Sample
source_name
MCF10A expressing satb1 fusion
cell line
MCF10A
cell type
human mammary gland epithelial cell line
genotype/variation
expressing Satb1 without homeodomain (HD)
chip antibody
Anti-GFP antibody (Abcam, Ab290)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells are fixed, washed, and lysed to purify the nuceli. Then chromatin were fragmented with Covaris, and mixed with antibody and beads to pull down the bound fraction as described in the manuscript Standard CHIP-seq libraries made using NEBnext Ultra II kit from chipped DNA