GSM3499112: MCF7-ARID1A KO Clone 14 ER Vehicle rep 2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
ESR1
Cell type
Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
MCF7-ARID1A KO Clone 14_ER ChIP-seq_Vehicle
cell line
MCF7
cell type
ER+ Breast Cancer Cell Line
passages
35-45
treatment
Vehicle
chip antibody
Abcam ab3575 and Millipore 06-935 antibody mix
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Nuclear lysates were prepared and antibody-bound chromatin was purified using appropriate antibodies. Antibodies used: ARID1A HPA005456, BRG1 ab215998, SNF5 Bethyl A301-087A, ERα ab3575 and Merck Millipore 06-935 antibody mix, H3K27ac Diagenode C15410196 Premium, HDAC1 Diagenode Premium C15410325, ARID2 Bethyl A302-229A, BRD4 from Cheng Ming Chiang's lab. DNA was prepared for Illumina sequencing on the HiSeq 4000 using the ThruPLEX DNA-seq kit according to the manufacturer's instructions.