GSM3495147: HepG2 C/EBPβ enh-/- BRD4 ChIPseq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
TFs and others
Cell type Class
Attributes by original data submitter
BRD4 (39909, Lot#24317004, Active Motif)
Sequenced DNA Library
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. After recovery of ChIP and input DNA, whole-genome amplification was performed using the WGA4 kit (Sigma-Aldrich) and BpmI-WGA primers. Amplified DNA was digested with BpmI (New England Biolab). After that, 30 ng of the amplified DNA was used with the NEBNext ChIP-seq library prep reagent set (New England Biolab).