GSM3495145: HepG2 WT C/EBPB ChIPseq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
TFs and others
Cell type Class
Attributes by original data submitter
C/EBPB (sc-150, Lot#G1814, Santa Cruz Biotechnology)
Sequenced DNA Library
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. After recovery of ChIP and input DNA, whole-genome amplification was performed using the WGA4 kit (Sigma-Aldrich) and BpmI-WGA primers. Amplified DNA was digested with BpmI (New England Biolab). After that, 30 ng of the amplified DNA was used with the NEBNext ChIP-seq library prep reagent set (New England Biolab).