GSM1361784: mutant PAS preincubation; Drosophila melanogaster; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Adult
Cell type
Ovary
NA
NA
Attributes by original data submitter
Sample
source_name
mutant_mutant_PAS preincubation
strain
piwiNt/piwi2 (mutant)
age of animals
1-5 day old
tissue
Ovaries
sample type
Chromatin bound to Protein-A-sepharose without antibody
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Antibodies were used to perform ChIP and resulting chromatin fragments were used to prepare deep sequencing libraries. Please see treatment protocol for antibodies used. Libraries were prepared with NEBNext ChIP-Seq Sample Prep Master Mix Set 1 (NEB# E6240S) which provides in-line barcodes and libraries were sequenced with Illumina HiSeq 2000. Single-end sequencing of reads were up to 50 bases.