Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Su(var)205

Cell type

Cell type Class
Adult
Cell type
Ovary
NA
NA

Attributes by original data submitter

Sample

source_name
WT_WT_anti-HP1
strain
piwi/+ (wildtype)
age of animals
1-5 day old
tissue
Ovaries
chip antibody
anti-HP1
chip antibody vendor
Covance innovative

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Antibodies were used to perform ChIP and resulting chromatin fragments were used to prepare deep sequencing libraries. Please see treatment protocol for antibodies used. Libraries were prepared with NEBNext ChIP-Seq Sample Prep Master Mix Set 1 (NEB# E6240S) which provides in-line barcodes and libraries were sequenced with Illumina HiSeq 2000. Single-end sequencing of reads were up to 50 bases.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

dm3

Number of total reads
5750891
Reads aligned (%)
93.0
Duplicates removed (%)
24.7
Number of peaks
976 (qval < 1E-05)

dm6

Number of total reads
5750891
Reads aligned (%)
92.6
Duplicates removed (%)
27.5
Number of peaks
1892 (qval < 1E-05)

Base call quality data from DBCLS SRA