Antigen

Antigen Class

TFs and others

Antigen

Epitope tags

Cell type

Cell type Class

Adult

Cell type

Adult

NA

NA

Sample

source_name

Whole animals

strain

OP201

developmental stage

Day 1 adults

genotype

unc-119(tm4063); wgIs201

chip antibody

anti-FLAG (M2, Sigma-Aldrich, F1804)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Animals were harvested in M9 buffer, washed, and ground to a find powder with a mortar and pestle in liquid Nitrogen. Whole animal lysates were crosslinked with 1% formaldehyde for 5 minutes, and the genomic DNA was sheared using a Covaris E220 (Covaris). The resulting chromatin was immunoprecipitated with anti-HA or anti-FLAG antibodies. ChIP-Seq libraries were prepared for sequencing using the ThruPLEX DNA-Seq Kit (Rubicon Genomics) according to the manufacturer's instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

ce11

Number of total reads

25794530

Reads aligned (%)

93.8

Duplicates removed (%)

31.4

Number of peaks

1543 (qval < 1E-05)

ce10

Number of total reads

25794530

Reads aligned (%)

93.8

Duplicates removed (%)

31.4

Number of peaks

1551 (qval < 1E-05)