SRX5056011
GSM3486886: Pu-1 Irf8 shRNA1 ResLSD1i; Mus musculus; ChIP-Seq

Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Spi1

Cell type

Cell type Class
Blood
Cell type
Hematopoietic progenitors
NA
NA

Attributes by original data submitter

Sample

source_name
MLL-AF9 I-BET resistant cells
strain background
C57BL/6
tissue source
haematopoietic progenitors
cell line
MLL-AF9
cell phenotype
I-BET Resistant
shRNA
Irf8 shRNA
treatment
LSD1i
chip antibody
PU.1/SPI1(2258S, Cell Signaling)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysate were isolated from formaldehybe cross-linked cells and sonicated to release the chromatin, then immunoprecipitated with antibody ThruPLEXTM-FD Prep Kit protocol (Rubicon Genomics)

Sequencing Platform

instrument_model
NextSeq 500

Where can I get the processing logs?

Read processing pipeline log

mm10

Number of total reads
11147546
Reads aligned (%)
48.3
Duplicates removed (%)
11.0
Number of peaks
13949 (qval < 1E-05)

mm9

Number of total reads
11147546
Reads aligned (%)
48.2
Duplicates removed (%)
11.2
Number of peaks
13933 (qval < 1E-05)

Base call quality data from DBCLS SRA