Antigen

Antigen Class

TFs and others

Antigen

Irf8

Cell type

Cell type Class

Blood

Cell type

Hematopoietic progenitors

NA

NA

Sample

source_name

MLL-AF9 I-BET resistant cells

strain background

C57BL/6

tissue source

haematopoietic progenitors

cell line

MLL-AF9

cell phenotype

I-BET Resistant

shRNA

N/A

treatment

LSD1i

chip antibody

anti-Irf8 (5628S, Cell signaling)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysate were isolated from formaldehybe cross-linked cells and sonicated to release the chromatin, then immunoprecipitated with antibody ThruPLEXTM-FD Prep Kit protocol (Rubicon Genomics)

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

16591874

Reads aligned (%)

70.7

Duplicates removed (%)

19.9

Number of peaks

476 (qval < 1E-05)

mm9

Number of total reads

16591874

Reads aligned (%)

70.6

Duplicates removed (%)

20.0

Number of peaks

455 (qval < 1E-05)