Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Blood
Cell type
Hematopoietic progenitors
NA
NA

Attributes by original data submitter

Sample

source_name
MLL-AF9 I-BET resistant cells
strain background
C57BL/6
tissue source
haematopoietic progenitors
cell line
MLL-AF9
cell phenotype
I-BET Resistant
shRNA
Pu.1 Scrambled
treatment
DMSO
chip antibody
anti-H3K27ac (ab4729, Abcam)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysate were isolated from formaldehybe cross-linked cells and sonicated to release the chromatin, then immunoprecipitated with antibody ThruPLEXTM-FD Prep Kit protocol (Rubicon Genomics)

Sequencing Platform

instrument_model
NextSeq 500

mm10

Number of total reads
24767957
Reads aligned (%)
97.2
Duplicates removed (%)
8.2
Number of peaks
27038 (qval < 1E-05)

mm9

Number of total reads
24767957
Reads aligned (%)
97.1
Duplicates removed (%)
8.2
Number of peaks
27041 (qval < 1E-05)

Base call quality data from DBCLS SRA