Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Blood
Cell type
Natural killer cells
NA
NA

Attributes by original data submitter

Sample

source_name
CD27+CD11b+ NK cells
cell type
DP NK
sample type
Rep2
antibody
H3K27ac (abcam 4729)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from MNase treated nuclei and histone-DNA complexes were isolated with antibody. ATAC-seq were processed as per manufacturers instructions (Illumina FC-121-1030). RNA was purified using Rneasy Plus Microkit, and prepared using SMARTer Universal Low Input RNA kit (Clontech) Nextera DNA Library Preparation Kit, Illuminia

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
22690363
Reads aligned (%)
99.2
Duplicates removed (%)
19.7
Number of peaks
21097 (qval < 1E-05)

mm9

Number of total reads
22690363
Reads aligned (%)
99.0
Duplicates removed (%)
19.7
Number of peaks
21114 (qval < 1E-05)

Base call quality data from DBCLS SRA