GSM3483988: CD27 SP mouse NK Rep1 K27ac; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K27ac
Cell type
Cell type Class
Blood
Cell type
Natural killer cells
NA
NA
Attributes by original data submitter
Sample
source_name
CD27+CD11b- NK cells
cell type
CD27 SP NK
sample type
Rep1
antibody
H3K27ac (abcam 4729)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from MNase treated nuclei and histone-DNA complexes were isolated with antibody. ATAC-seq were processed as per manufacturers instructions (Illumina FC-121-1030). RNA was purified using Rneasy Plus Microkit, and prepared using SMARTer Universal Low Input RNA kit (Clontech) Nextera DNA Library Preparation Kit, Illuminia