Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Neural
Cell type
Neural Stem Cells
MeSH Description
Self-renewing cells that generate the main phenotypes of the nervous system in both the embryo and adult. Neural stem cells are precursors to both NEURONS and NEUROGLIA.

Attributes by original data submitter

Sample

source_name
30 hr differentiation Neural Stem Cells
cell type
SVZ derived neural stem cells
passages
5 to 7
strain
C57BL/6
chip antibody
None

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared using standard Illumina protocols

Sequencing Platform

instrument_model
Illumina Genome Analyzer

mm10

Number of total reads
123207056
Reads aligned (%)
88.7
Duplicates removed (%)
25.1
Number of peaks
544 (qval < 1E-05)

mm9

Number of total reads
123207056
Reads aligned (%)
88.1
Duplicates removed (%)
24.7
Number of peaks
619 (qval < 1E-05)

Base call quality data from DBCLS SRA