Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Neural

Cell type

Neural Stem Cells

MeSH Description

Self-renewing cells that generate the main phenotypes of the nervous system in both the embryo and adult. Neural stem cells are precursors to both NEURONS and NEUROGLIA.

Sample

source_name

30 hr differentiation Neural Stem Cells

cell type

SVZ derived neural stem cells

passages

5 to 7

strain

C57BL/6

chip antibody

H3K27me3 Millipore 07-449

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared using standard Illumina protocols

Sequencing Platform

instrument_model

Illumina Genome Analyzer

mm10

Number of total reads

86708574

Reads aligned (%)

88.7

Duplicates removed (%)

40.9

Number of peaks

780 (qval < 1E-05)

mm9

Number of total reads

86708574

Reads aligned (%)

88.2

Duplicates removed (%)

40.7

Number of peaks

831 (qval < 1E-05)