Curated Sample Data


Genome
ce10
Antigen Class
Histone
Antigen
H3K4me3
Cell type Class
Larvae
Cell type
L3

Cell type information


NA
NA

Attributes by Original Data Submitter


source_name
whole worms
strain
15eh1
developmental stage
L3
antibody target
H3K4me3
antibody antigen
Human Histone H3 aa 1-100 (tri methyl K4) conjugated to Keyhole Limpet Haemocyanin (KLH)
matching input
SEA152_input_15eh1_L3

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Embryos were washed and dounced in FA buffer (50 mM HEPES/KOH pH 7.5, 1 mM EDTA, 1% Triton X-100, 0.1 % sodium deoxycholate; 150 mM NaCl). 0.1 sarkosyl was added before sonicating to obtain chromatin fragments of majority between 200-800 bp. 1-2 mg of embryo extract and 3-5 ug of antibody was used per ChIP. Half of the ChIP DNA were ligated to Illumina or home-made multiplexed adapters and amplified by PCR. Library DNA between 250-500 bp in size was gel purified.

Platform Information


instrument_model
Illumina HiSeq 2500

External Database Query

Logs in read processing pipeline


Number of total reads
7236232
Reads aligned (%)
95.0
Duplicates removed (%)
5.8
Number of peaks
2691 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA