Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
dpy-27

Cell type

Cell type Class
Larvae
Cell type
L3
NA
NA

Attributes by original data submitter

Sample

source_name
whole worms
strain
15eh1
developmental stage
L3
antibody target
DPY-27
antibody antigen
1-409 aa
matching input
SEA152_input_15eh1_L3

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Embryos were washed and dounced in FA buffer (50 mM HEPES/KOH pH 7.5, 1 mM EDTA, 1% Triton X-100, 0.1 % sodium deoxycholate; 150 mM NaCl). 0.1 sarkosyl was added before sonicating to obtain chromatin fragments of majority between 200-800 bp. 1-2 mg of embryo extract and 3-5 ug of antibody was used per ChIP. Half of the ChIP DNA were ligated to Illumina or home-made multiplexed adapters and amplified by PCR. Library DNA between 250-500 bp in size was gel purified.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

ce10

Number of total reads
5640309
Reads aligned (%)
73.3
Duplicates removed (%)
12.4
Number of peaks
1540 (qval < 1E-05)

ce11

Number of total reads
5640309
Reads aligned (%)
73.3
Duplicates removed (%)
12.4
Number of peaks
1561 (qval < 1E-05)

Base call quality data from DBCLS SRA