Curated Sample Data


Genome
ce10
Antigen Class
TFs and others
Antigen
capg-1
Cell type Class
Embryo
Cell type
Mixed embryo

Cell type information


NA
NA

Attributes by Original Data Submitter


source_name
whole worms
strain
GW638
developmental stage
mixed embryo
antibody target
CAPG-1
antibody antigen
1-100 aa
matching input
LAS52_input_GW638_emb

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Embryos were washed and dounced in FA buffer (50 mM HEPES/KOH pH 7.5, 1 mM EDTA, 1% Triton X-100, 0.1 % sodium deoxycholate; 150 mM NaCl). 0.1 sarkosyl was added before sonicating to obtain chromatin fragments of majority between 200-800 bp. 1-2 mg of embryo extract and 3-5 ug of antibody was used per ChIP. Half of the ChIP DNA were ligated to Illumina or home-made multiplexed adapters and amplified by PCR. Library DNA between 250-500 bp in size was gel purified.

Platform Information


instrument_model
NextSeq 500

External Database Query

Logs in read processing pipeline


Number of total reads
12014281
Reads aligned (%)
84.1
Duplicates removed (%)
13.2
Number of peaks
1543 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA